| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;W3/25 antigen;CD4;Cd4; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Anti-CD4 Antibody (Monoclonal, CA-4) is an antibody targeting CD4. Common applications include IHC, ICC, Flow Cytometry. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: CA-4; isotype: Mouse IgG1; reactivity: Human; observed MW: 25 kDa; calculated MW: 51438 MW.
Boster Bio Anti-CD4 Antibody (Monoclonal, CA-4) catalog # MA1016. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CD4 — T-cell surface glycoprotein CD4
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: CA-4; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 25 kDa; Calculated: 51438 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
Scientific background (datasheet): CD stands for cluster of differentiation; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Cellular localization (datasheet): Membrane; Single-pass type I membrane protein.
Tissue details (datasheet): Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .
Sequence similarities (datasheet): Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,Antiviral Signaling,Hematopoietic Progenitors,Immune System Diseases,Immunology,Lymphoid,Myeloid,Stem Cells,T Cells,T Lymphocytic Lineage.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
