| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;W3/25 antigen;CD4;Cd4; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CA-4 is a mouse monoclonal antibody that recognizes T-cell surface glycoprotein CD4 in human samples and is validated for IHC, ICC/IF and flow cytometry.
CD4 is a transmembrane glycoprotein on helper T cells, and also on some monocytes and macrophages, that acts as a co-receptor for MHC class II–peptide complexes. It recruits the kinase LCK to boost T-cell receptor signaling, and it is the primary receptor used by HIV-1 to enter cells.
| Target | T-cell surface glycoprotein CD4 (gene Cd4; UniProt P05540, rat) |
|---|---|
| Clone | CA-4 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (frozen sections) | 1 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Flow cytometry | 1–3 µg/1×106 cells (Human) |
Immunogen
CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
Reactivity Notes
The supplier lists reactivity with human. UniProt places T-cell surface glycoprotein CD4 in the cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
