| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-IIb;GPalpha IIb;GPIIb;Platelet membrane glycoprotein IIb;CD41;Integrin alpha-IIb heavy chain;Integrin alpha-IIb light chain, form 1;Integrin alpha-IIb light chain, form 2;ITGA2B;GP2B, ITGAB; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD41 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAGH-9 is a rabbit monoclonal antibody that recognizes integrin alpha-IIb (gene ITGA2B) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 113.4 kDa, and the supplier reports an observed band at about 130 kDa.
Integrin alpha-IIb (ITGA2B, CD41) pairs with integrin beta-3 to form the platelet fibrinogen receptor αIIbβ3 (GPIIb/IIIa). When platelets are activated, the integrin shifts to a high-affinity state and binds fibrinogen and von Willebrand factor, linking platelets into aggregates. Defects cause Glanzmann thrombasthenia, and CD41 is a standard platelet and megakaryocyte marker.
| Target | Integrin alpha-IIb (gene ITGA2B; UniProt P08514, human) |
|---|---|
| Clone | AAGH-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 113.4 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): rat pancreas tissue, human squamous carcinoma tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CD41.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin alpha-IIb in the cell membrane. Tissue expression noted by UniProt (human): Isoform 1 and isoform 2 are expressed in platelets and megakaryocytes, but not in reticulocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Runfeng Ni et al. (2022) A mouse model of irradiation and spleen-thymus lymphocyte infusion induced aplastic anemia. Hematology. 10.1080/16078454.2022.2113356
