Anti-CD82 Monoclonal Antibody

SKU:BHA21008913
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Boster Bio
Boster Bio
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Overview
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Anti-CD82 antibody from Rabbit (Monoclonal, clone ADOB-3, isotype Rabbit IgG). Commonly used in Immunology & Inflammation research; including WB, IHC, Flow applications.
Target CD82
clone number ADOB-3
Host Rabbit
Reactivity Human,Rat
Isotype Rabbit IgG
Application(s) WB, IHC, Flow
Options selector
Catalog no. Size Conjugation
M02300 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC,Flow Cytometry
      Application details: WB 1:500-2000<br>IHC 1:50-200<br>FC 1:20<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No M02300
Alternative Names Solute carrier family 2, facilitated glucose transporter member 2;Glucose transporter type 2, liver;GLUT-2;SLC2A2;GLUT2;
Cellular Localization Membrane; Multi-pass membrane protein.
Clonality
  • Monoclonal
Concentration 0.5mg/ml
Form Liquid
Host Rabbit
Immunogen A synthesized peptide derived from human CD82 Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Isotype
  • Rabbit IgG
Molecular Weight 38 kDa
Product Type
  • Antibodies
  • Primary Antibodies
Reactivity
  • Human
  • Rat
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Target CD82
UniProt # P27701

Overview

This product is an anti-CD82 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADOB-3; isotype Rabbit IgG; reactivity: Human,Rat. Reported application contexts include WB, IHC, Flow (as provided in the source record). Boster Bio Anti-CD82 Monoclonal Antibody catalog # M02300. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Rat.

Key elements and design rationale

  • Target: CD82 (Solute carrier family 2, facilitated glucose transporter member 2).
  • Antibody format: Monoclonal; clone ADOB-3; isotype Rabbit IgG.
  • Host: Rabbit.
  • Species reactivity: Human,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

CD82 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Facilitative glucose transporter. This isoform likely mediates the biional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na (+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Reported cellular localization context: Membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Liver, insulin-producing beta cell, small intestine and kidney.

Research relevance and current trends

  • Research context keywords from the source record include: Adaptive Immunity,Cancer,Cancer Susceptibility,Cell Type Markers,Epigenetics and Nuclear Signaling,Immunology,NK Cells,Oncoproteins/Suppressors,Platelets,T Cells,Transcription,Tumor Suppressors.
  • Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
  • Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

  • Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
  • Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
  • Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate CD82 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CD82 expression by Western blot in cell or tissue lysates, Detect CD82 in FFPE tissue sections by immunohistochemistry, Quantify CD82-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

  • Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
  • Apparent molecular weight may vary by sample type and processing (observed MW: 38 kDa; calculated MW: 57490 MW).
  • Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

  • Molecular weight (observed): 38 kDa
  • Cellular localization (provided): Membrane; Multi-pass membrane protein.
  • Tissue details (provided): Liver, insulin-producing beta cell, small intestine and kidney.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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