| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-CD9 (extracellular)-FITC Antibody is an antibody targeting Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3 Polyclonal raised in Rabbit (Fluorescein isothiocyanate (FITC)). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3 (also reported as Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3).
- Immunogen/epitope region: Extracellular, 2nd loop..
- Homology note: Rat - identical, Human - 14 out of 16 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis (unlabeled antibody, #ANR-209), and direct flow cytometry (labeled antibody)..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Fluorescein isothiocyanate (FITC) (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Cluster of Differentiation 9 (CD9), also known as Tetrapanin-29, TSPAN29 and Leukocyte Antigen MIC3, is a member of the tetraspanin superfamily, a group of proteins characterized by four transmembrane domains, two extracellular loops, and N- and C- termini cytoplasmic tails.1The tetraspanins family members function as organizers of the cell surface by recruiting specific partner proteins into tetraspanin-enriched microdomains. Thus, depending on the recruited protein, they influence many cellular activities in different cells, including intracellular signaling, proliferation, activation, survival, migration, invasion, adhesion, and diapedesis.1CD9 was originally identified as a lymphohematopoietic marker, and is indeed expressed in all the major subsets of leukocytes, however, it is also widely expressed across different cell populations including endothelial cells, neurons, glia and oocytes.2,3CD9 plays various roles in the immune system, it has been implicated in T cell activation and participating in the formation of immunological synapses by interacting with the integrin LFA-1 (on the T-cell side) and their ligand, the integrin ICAM-1 on the antigen presenting cell (APC) surface.2,3 In B cells, CD9 plays a role in B cell activation and regulation of antibody production, and in mast cells is involved in the regulation of signalling pathways that influence the release of allergic mediators in response to allergens.2,3CD9 has complex roles in the central nervous system (CNS). It has been implicated in neurite outgrowth and cell migration in association with the neural adhesion molecule L1 (L1CAM).
Research relevance and current trends
- Profiling immune-marker expression across cell subsets with single-cell or flow-based readouts.
- Connecting receptor/ligand levels to activation state and cytokine programs.
- Applying genetic perturbation or orthogonal assays to support specificity and interpretation.
Common research applications
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: RIC-001-F.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-NR209; Negative control: RIC-001-F.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
