Anti-CDK12 Antibody Picoband®

Overview

Anti-CDK12 Antibody Picoband® is an antibody for CDK12 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA, IP workflows.

Key elements and design rationale

• Target: CDK12 (Calpain-2 catalytic subunit); UniProt: Q9NYV4
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 205 kDa, calculated 79995 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA, IP

Vendor description (summary): Boster Bio Anti-CDK12 Antibody Picoband® catalog # A03495-1.

Biological background

Biological context: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). .

Expression and localization notes: cellular localization: Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca (2+) binding., tissue context: Ubiquitous..

Common research applications

• Western blotting (WB): Compare CDK12 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CDK12 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify CDK12-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: CDK12 cyclin-dependent kinase 12 is a protein kinase that in humans is encoded by the CDK12 gene. This enzyme is a member of cyclin-dependent kinase protein family. CDK12 forms protein complexes with cyclin K (CCNK). The CDK12-CCNK complex acts at the level of RNA polymerase II (RNAPII) to regulate expression of long complex genes, several of which have roles in DNA maintenance and repair。
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca (2+) binding.
• Tissue details: Ubiquitous.
• Research category: Calcium Signaling,Cancer,Cancer Metabolism,Cell Biology,Metabolism,Metabolism Processes,Pathways and Processes,Proteolysis/Ubiquitin,Proteolytic Enzymes,Response To Hypoxia,Signal Transduction,Signaling Pathway

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.