| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cyclin-dependent kinase 6 ;Protein Cdk6 ;Cdk6 ;rCG_41083 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant human Cdk6 protein. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CDK6 Antibody (Monoclonal, DCS-90) is an antibody targeting CDK6. Common applications include WB, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: DCS-90; isotype: Mouse IgG1; reactivity: Human,Mouse,Rat; observed MW: 37 kDa; calculated MW: 36952 MW.
Boster Bio Anti-CDK6 Antibody (Monoclonal, DCS-90) catalog # MA1021. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CDK6 — cyclin-dependent kinase 6
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: DCS-90; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 37 kDa; Calculated: 36952 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): CDK6: Serine/threonine-protein kinase involved in the controlof the cell cycle and differentiation; promotes G1/S transition.Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclinsduring interphase at G1 to form a pRB/RB1 kinase and controls theentrance into the cell cycle. Involved in initiation andmaintenance of cell cycle exit during cell differentiation;prevents cell proliferation and regulates negatively celldifferentiation, but is required for the proliferation of specificcell types (e.g. erythroid and hematopoietic cells). Essential forcell proliferation within the dentate gyrus of the hippocampus andthe subventricular zone of the lateral ventricles. Required duringthymocyte development. Promotes the production of newborn neurons,probably by modulating G1 length. Promotes, at least inastrocytes, changes in patterns of gene expression, changes in theactin cytoskeleton including loss of stress fibers, and enhancedmotility during cell differentiation. Prevents myeloiddifferentiation by interfering with RUNX1 and reducing itstranscription transactivation activity, but promotes proliferationof normal myeloid progenitors. Delays senescence. Promotes theproliferation of beta-cells in pancreatic islets of Langerhans.May play a role in the centrosome organization during the cellcycle phases (PubMed:23918663).
Scientific background (datasheet): The cyclin-dependent protein kinases (CDKs) regulate major cell cycle transitions in eukaryotic cells. Cyclin-dependent kinase-6 (CDK6) is the earliest inducible member of the CDK family in human T lymphocytes, involved in growth factor stimulation and cell cycle progression. Germline mutations in CDK6 do not make a significant contribution to melanoma predisposition. CDK6 gene is mapped at 7q21-q22.
Cellular localization (datasheet): CDK6: Cytoplasm. Nucleus. Cell projection, ruffle.Cytoplasm, cytoskeleton, microtubule organizing center, centrosome|Cdk6: Cytoplasm
Tissue details (datasheet): CDK6: Expressed ubiquitously. Accumulates insquamous cell carcinomas, proliferating hematopoietic progenitorcells, beta-cells of pancreatic islets of Langerhans, andneuroblastomas. Reduced levels in differentiating cells.
Sequence similarities (datasheet): Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cell Biology,Cell Cycle,Epigenetics and Nuclear Signaling,Kinases/Phosphatases.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
