| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase 6;2.7.11.22;Cell division protein kinase 6;Serine/threonine-protein kinase PLSTIRE;CDK6;CDKN6; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CDK6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HOA-3 is a rabbit monoclonal antibody that recognizes cyclin-dependent kinase 6 (gene CDK6) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 36.9 kDa, and the supplier reports an observed band at about 37 kDa.
CDK6 is a serine/threonine kinase that forms complexes with D-type cyclins to phosphorylate RB1 and move cells from G1 into S phase. Besides its cell cycle role it influences differentiation, particularly in blood cell development, and it is a key target in cancer research alongside CDK4.
| Target | Cyclin-dependent kinase 6 (gene CDK6; UniProt Q00534, human) |
|---|---|
| Clone | HOA-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.9 kDa |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human THP-1 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:5000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): K562 cells.
Immunogen
Synthetic peptide based on the sequence of human CDK6.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cyclin-dependent kinase 6 in the cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (human): Expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Zhang et al. (2026) ERK promotes miR34a/ISOC1 signaling to enhance all trans retinoic acid-induced myeloid differentiation in acute promyelocytic leukemia. Journal of Biological Chemistry. 10.1016/j.jbc.2026.113284
Yueling Tang et al. (2023) Role and mechanism of Circ-PDE7B in the formation of keloid. International Wound Journal. 10.1111/iwj.14269
