| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen; CEA; Meconium antigen 100; CD66e; CEACAM5; CEA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CEACAM5 recombinant protein (Position: K35-N650). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cell adhesion molecule CEACAM5 in human, mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 76.8 kDa, and the supplier reports an observed band at about 130–200 kDa.
CEACAM5, better known as carcinoembryonic antigen (CEA, CD66e), is a GPI-anchored glycoprotein of the immunoglobulin superfamily. It mediates cell adhesion with itself and related CEACAMs, and because it is often highly expressed in colorectal and other carcinomas, it is one of the most widely studied tumor-associated antigens.
| Target | Cell adhesion molecule CEACAM5 (gene CEACAM5; UniProt P06731, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 76.8 kDa |
| Observed MW | 130–200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SW620 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human intestinal cancer tissue, human lung cancer tissue, mouse intestine tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CEACAM5 fragment (Lys35–Asn650), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cell adhesion molecule CEACAM5 in the cell membrane, apical cell membrane and cell surface. Tissue expression noted by UniProt (human): Expressed in columnar epithelial and goblet cells of the colon (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xin Zhang et al. (2022) Band-Edge Effect-Induced Electrochemiluminescence Signal Amplification Based on Inverse Opal Photonic Crystals for Ultrasensitive Detection of Carcinoembryonic Antigen. Analytical Chemistry. 10.1021/acs.analchem.2c01986
Zhengjun Wu et al. (2014) An aptasensor for carcinoembryonic antigen based on upconversion fluorescence resonance energy transfer. Sensors and Actuators B-Chemical. 10.1016/j.snb.2014.09.084
