| Field | Specification |
|---|---|
| Target | |
| Alternative names | CCAAT/enhancer-binding protein beta ;C/EBP beta ;Liver activator protein;LAP;Liver-enriched inhibitory protein;LIP;Nuclear factor NF-IL6;Transcription factor 5;TCF-5;CEBPB ;TCF5;PP9092; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CEBP Beta recombinant protein (Position: M1-A200). Human CEBP Beta shares 61% amino acid (aa) sequence identity with both mouse and rat CEBP Beta. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects CCAAT/enhancer-binding protein beta (gene CEBPB) in human samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 36.1 kDa; the supplier reports observed bands at 42, 46 kDa.
C/EBPβ (CEBPB) is a basic leucine zipper transcription factor that controls many immune and inflammatory genes, including those induced by IL-6 and IL-1. It also regulates adipogenesis, liver metabolism and regeneration, and its isoforms made from alternative start sites (LAP and LIP) have opposing activities.
| Target | CCAAT/enhancer-binding protein beta (gene CEBPB; UniProt P17676, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 36.1 kDa |
| Observed MW | 42, 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue, human squamous metaplasia of renal pelvis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human CEBP Beta fragment (Met1–Ala200), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 61%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places CCAAT/enhancer-binding protein beta in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed at low levels in the lung, kidney and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ma Cui et al. (2026) Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate. Cellular and Molecular Life Sciences. 10.1007/s00018-025-06071-3
Qiwei Liu et al. (2023) Convergent alteration of the mesenchymal stem cell heterogeneity in adipose tissue during aging. FASEB Journal. 10.1096/fj.202300807R
Na Wang et al. (2019) IRS-1 targets TAZ to inhibit adipogenesis of rat bone marrow mesenchymal stem cells through PI3K-Akt and MEK-ERK pathways. European Journal of Pharmacology. 10.1016/j.ejphar.2019.01.064
Koto Ukon et al. (2024) Spatial transcriptome analysis of lung squamous cell carcinoma arising from interstitial pneumonia provides insights into tumor heterogeneity. Pathology Research and Practice. 10.1016/j.prp.2024.155805
