| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Trem-like transcript 1 protein;TLT-1;Triggering receptor expressed on myeloid cells-like protein 1;TREML1;TLT1;UNQ1825/PRO3438; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse CINP, which shares 85.7% and 100% amino acid (aa) sequence identity with human and rat CINP, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CINP Antibody Picoband® is an antibody for Cinp detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Cinp (Trem-like transcript 1 protein); UniProt: Q9D0V8
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 26 kDa, calculated 32679 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CINP Antibody Picoband® catalog # A07687-1.
Biological background
Biological context: Cell surface receptor that may play a role in the innate and adaptive immune response. .
Expression and localization notes: cellular localization: Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Sequestered in cytoplasmic vesicles in resting platelets (PubMed:15100151). Transported to the cell surface after stimulation by thrombin (PubMed:15100151). Soluble fragments can be released into the serum by proteolysis (PubMed:16505478)., tissue context: Detected in platelets, monocytic leukemia and in T-cell leukemia. ..
Common research applications
- Western blotting (WB): Compare Cinp levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Cinp in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Cinp-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Cartilage intermediate layer protein 1 is a protein that in humans is encoded by the CILP gene. The protein encoded by this gene is reported to be a component of the DNA replication complex as well as a genome-maintenance protein. It may interact with proteins important for replication initiation and has been shown to bind chromatin at the G1 phase of the cell cycle and dissociate from chromatin with replication initiation. It may also serve to regulate checkpoint signaling as part of the DNA damage response. Alternative splicing results in multiple transcript variants.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Sequestered in cytoplasmic vesicles in resting platelets (PubMed:15100151). Transported to the cell surface after stimulation by thrombin (PubMed:15100151). Soluble fragments can be released into the serum by proteolysis (PubMed:16505478).
- Tissue details: Detected in platelets, monocytic leukemia and in T-cell leukemia. .
- Research category: Cardiovascular,Immunology,Innate Immunity,Macrophage/Inflammation,Platelets,Protein Phosphorylation,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
