| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cold-inducible RNA-binding protein; A18 hnRNP; Glycine-rich RNA-binding protein CIRP; CIRBP; A18HNRNP; CIRP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from CIRBP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26C38 is a rabbit monoclonal antibody that recognizes cold-inducible RNA-binding protein (gene CIRBP) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 18.6 kDa, and the supplier reports an observed band at about 19 kDa.
Cold-inducible RNA-binding protein (CIRBP) is an RNA-binding protein whose expression rises when cells are cooled or stressed. It binds mRNAs to stabilize them and influence their translation, and when released from cells it can act as a damage-associated signal that promotes inflammation.
| Target | Cold-inducible RNA-binding protein (gene CIRBP; UniProt Q14011, human) |
|---|---|
| Clone | 26C38 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 18.6 kDa |
| Observed MW | 19 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human RT4 cells, human U251 cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human colon tissue, human pancreas cancer tissue, mouse brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of CIRBP.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places cold-inducible RNA-binding protein in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Lei et al. (2017) Cold-inducible ribonucleic acid-binding protein attenuates acute kidney injuries after deep hypothermic circulatory arrest in rats. Interactive Cardiovascular and Thoracic Surgery. 10.1093/icvts/ivx262
