| Field | Specification |
|---|---|
| Target | |
| Alternative names | H; +; /Cl; -; exchange transporter 5; Chloride channel protein 5; ClC-5; Chloride transporter ClC-5; CLCN5; CLCK2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CLCN5 recombinant protein (Position: R100-S756). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets H(+)/Cl(-) exchange transporter 5 (gene CLCN5) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 83.1 kDa, and the supplier reports an observed band at about 100 kDa.
ClC-5 (CLCN5) is a CLC family 2Cl−/H+ exchanger found mainly in endosomes of kidney proximal tubule cells. It supports endosomal function needed for protein reabsorption from the filtrate, and mutations in CLCN5 cause Dent disease.
| Target | H(+)/Cl(-) exchange transporter 5 (gene CLCN5; UniProt P51795, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 83.1 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, rat liver tissue, rat heart tissue, rat kidney tissue, mouse brain tissue, mouse liver tissue, mouse heart tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human CLCN5 fragment (Arg100–Ser756), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places H(+)/Cl(-) exchange transporter 5 in the Golgi apparatus membrane, endosome membrane and cell membrane. Tissue expression noted by UniProt (human): Kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
