Anti-ClC-5/CLCN5 Antibody Picoband®

Overview

Anti-ClC-5/CLCN5 Antibody Picoband® is an antibody for CLCN5 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: CLCN5 (mannose receptor C-type 1); UniProt: P51795
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 100 kDa, calculated 75694 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-ClC-5/CLCN5 Antibody Picoband® catalog # A02286-2.

Biological background

Biological context: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. (Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens. (Microbial infection) Acts as a receptor for Dengue virus envelope protein E. (Microbial infection) Interacts with Hepatitis B virus envelope protein.

Expression and localization notes: cellular localization: Cell membrane. Single-pass type I membrane protein. Endosome membrane. Single-pass type I membrane protein., tissue context: Ubiquitous..

Common research applications

• Western blotting (WB): Compare CLCN5 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CLCN5 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify CLCN5-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: The CLCN5 gene encodes the chloride channel Cl-/H+ exchanger ClC-5. This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane. Single-pass type I membrane protein. Endosome membrane. Single-pass type I membrane protein.
• Tissue details: Ubiquitous.
• Research category: Cardiovascular,Cell Biology,Cell Cycle,Cell Differentiation,Lipases,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.