| Field | Specification |
|---|---|
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| Alternative Names | C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369 |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
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| Target |
Overview
Anti-CLEC7A/Dectin-1 (extracellular) Antibody is an antibody targeting C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, IHC, LCI, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369 (also reported as C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369).
- Immunogen/epitope region: Extracellular, C-terminus..
- Homology note: Rat - 15 out of 16 amino acid residues identical Not recommended for human samples (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Dectin-1, encoded by CLEC7A, is a small transmembrane receptor that is a member of the C-type lectin family of pattern-recognition receptors (PRRs)1,2. As this receptor specifically recognizes and binds to β-glucans polysaccharides derived from the cell walls of fungi and yeast, it has been characterized for its role in antifungal immunity3.Dectin-1 is predominately expressed on macrophages, dendritic cells, and neutrophils4,5. Upon ligation of β-glucan, Dectin-1 recruits the adaptor protein CARD9 to phosphorylate spleen tyrosine kinase (Syk) which in turn initiates a signaling cascade which induces phagocytosis and leads to the activation of nuclear factor-κB (NF-κB) and MAPK6.
Research relevance and current trends
- Profiling immune-marker expression across cell subsets with single-cell or flow-based readouts.
- Connecting receptor/ligand levels to activation state and cytokine programs.
- Applying genetic perturbation or orthogonal assays to support specificity and interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-LR062.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-LR062; Negative control: BLP-LR062.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
