| Field | Specification |
|---|---|
| Target | |
| Alternative names | CAP-Gly domain-containing linker protein 1; Cytoplasmic linker protein 1; Cytoplasmic linker protein 170 alpha-2; CLIP-170; Reed-Sternberg intermediate filament-associated protein; Restin; CLIP1; CYLN1; RSN |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CLIP170 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29C06 is a rabbit monoclonal antibody that recognizes CAP-Gly domain-containing linker protein 1 (gene CLIP1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 162.2 kDa, and the supplier reports an observed band at about 170 kDa.
CLIP-170 (CLIP1) is a microtubule plus-end tracking protein that binds growing microtubule ends and promotes their growth. It links endocytic vesicles and other cargo to microtubules and helps position dynein, so it is studied in microtubule dynamics, intracellular transport and cell polarity.
| Target | CAP-Gly domain-containing linker protein 1 (gene CLIP1; UniProt P30622, human) |
|---|---|
| Clone | 29C06 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 162.2 kDa |
| Observed MW | 170 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human SiHa cells, human A549 whoel cells, human MCF-7 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CLIP170.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places CAP-Gly domain-containing linker protein 1 in the cytoplasm, cytoplasmic vesicle membrane and cell projection. Tissue expression noted by UniProt (human): Detected in dendritic cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
