| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Collagen alpha-1 (XVIII) chain; Endostatin; COL18A1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human COL18A1 recombinant protein (Position: H1572-K1754). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of COL18A1 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-COL18A1 Antibody Picoband® catalog # A01302-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human COL18A1 recombinant protein (Position: H1572-K1754). (reported region: H1572-K1754).
- Molecular weight context: reported MW: 180 kDa, 20 kDa; calculated MW: nan
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
collagen, type XVIII, alpha 1. Collagen alpha-1 (XVIII) chain is a protein that in humans is encoded by the COL18A1 gene. This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. Functional note: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Reported localization: Secreted, extracellular space, extracellular matrix. Expression/tissue context: Present in multiple organs with highest levels in liver, lung and kidney.
Research relevance and current trends
- Angiogenesis: Researchers commonly examine how COL18A1 relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how COL18A1 relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how COL18A1 relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative COL18A1 levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
