| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (XVIII) chain; Endostatin; COL18A1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human COL18A1 recombinant protein (Position: H1572-K1754). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets collagen alpha-1(XVIII) chain (gene COL18A1) in human samples and is validated for Western blot. The predicted molecular weight is 178.2 kDa; the supplier reports observed bands at 180 kDa, 20 kDa.
Collagen α1(XVIII) chain (COL18A1) is a basement membrane proteoglycan-type collagen important for eye development and neural tube closure. Its C-terminal fragment, endostatin, inhibits endothelial cell migration and angiogenesis, so COL18A1 is studied in vascular biology, retinal development and cancer research.
| Target | Collagen alpha-1(XVIII) chain (gene COL18A1; UniProt P39060, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 178.2 kDa |
| Observed MW | 180 kDa, 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human COLO-320 cells, human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human COL18A1 fragment (His1572–Lys1754), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes collagen alpha-1(XVIII) chain as a secreted protein. Tissue expression noted by UniProt (human): Detected in placenta (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan Zhang et al. (2009) Combined endostatin and TRAIL gene transfer suppresses human hepatocellular carcinoma growth and angiogenesis in nude mice. Cancer Biology & Therapy. 10.4161/cbt.8.5.7687
Ma Jingxi et al. (2008) Effects of Electroacupuncture on Expressions of Angiogenesis Factors and Anti-angiogenesis Factors in Brain of Experimental Cerebral Ischemic Rats after Reperfusion. Journal of Traditional Chinese Medicine. 10.1016/S0254-6272(08)60050-3
