| Field | Specification |
|---|---|
| Target | |
| Alternative names | Coatomer subunit alpha; Alpha-coat protein; Alpha-COP; HEP-COP; HEPCOP; COPA |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human COPA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22C64 is a rabbit monoclonal antibody that recognizes coatomer subunit alpha (gene COPA) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 138.3 kDa, and the supplier reports an observed band at about 160 kDa.
COPA is the α subunit of coatomer, the protein complex that forms COPI coats on Golgi-derived vesicles. COPI vesicles mediate retrograde transport from the Golgi back to the endoplasmic reticulum and transport within the Golgi, and COPA mutations cause an autoimmune disorder known as COPA syndrome.
| Target | Coatomer subunit alpha (gene COPA; UniProt P53621, human) |
|---|---|
| Clone | 22C64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 138.3 kDa |
| Observed MW | 160 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human COPA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places coatomer subunit alpha in the cytoplasm and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Uniformly expressed in a wide range of adult and fetal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
