| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dual specificity mitogen-activated protein kinase kinase 5;MAP kinase kinase 5;MAPKK 5;2.7.12.2;MAPK/ERK kinase 5;MEK 5;MAP2K5;MEK5, MKK5, PRKMK5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human COPA |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-COPA antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22C64; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-COPA Rabbit Monoclonal Antibody catalog # M03987. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: COPA (Dual specificity mitogen-activated protein kinase kinase 5).
- Antibody format: Monoclonal; clone 22C64; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
COPA (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. . Reported cellular localization context: Cytoplasm, cytoskeleton . Cell projection, lamellipodium . At the interface between the lamellipodial actin meshwork and the membrane. . Tissue expression notes (as provided): Expressed in many adult tissues. Abundant in heart and skeletal muscle.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cardiogenesis,Cardiovascular,MAPK Pathway,Protein Phosphorylation,Ser/Thr Kinases,Serine/Threonine Kinases,Signal Transduction,Transcription Factors/Regulators.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate COPA antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect COPA expression by Western blot in cell or tissue lysates, Detect COPA in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 138 kDa; calculated MW: 50112 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 138 kDa
- Cellular localization (provided): Cytoplasm, cytoskeleton . Cell projection, lamellipodium . At the interface between the lamellipodial actin meshwork and the membrane. .
- Tissue details (provided): Expressed in many adult tissues. Abundant in heart and skeletal muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
