| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prostaglandin G/H synthase 2;1.14.99.1;Cyclooxygenase-2;COX-2;PHS II;Prostaglandin H2 synthase 2;PGH synthase 2;PGHS-2;Prostaglandin-endoperoxide synthase 2;PTGS2;COX2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cox2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFHD-16 is a rabbit monoclonal antibody that recognizes prostaglandin G/H synthase 2 (gene PTGS2) in human and mouse samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 69.0 kDa, and the supplier reports an observed band at about 75 kDa.
COX-2 (PTGS2) is the inducible cyclooxygenase-peroxidase that converts arachidonic acid into prostaglandin H2, the precursor of prostaglandins and thromboxanes. Its expression rises sharply in response to cytokines, growth factors and tumor promoters, making it a central marker in studies of inflammation, pain and cancer and the target of selective COX-2 inhibitors.
| Target | Prostaglandin G/H synthase 2 (gene PTGS2; UniProt P35354, human) |
|---|---|
| Clone | AFHD-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.0 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human cox2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places prostaglandin G/H synthase 2 in the microsome membrane, endoplasmic reticulum membrane, nucleus inner membrane and nucleus outer membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Siyu He et al. (2024) Superenhancer-driven circRNA Myst4 involves in pulmonary artery smooth muscle cell ferroptosis in pulmonary hypertension. iScience. 10.1016/j.isci.2024.110900
