| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Prostaglandin G/H synthase 2;1.14.99.1;Cyclooxygenase-2;COX-2;PHS II;Prostaglandin H2 synthase 2;PGH synthase 2;PGHS-2;Prostaglandin-endoperoxide synthase 2;PTGS2;COX2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Cox2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PTGS2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFHD-16; isotype Rabbit IgG; reactivity: Human,Mouse. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-Cox2 PTGS2 Monoclonal Antibody catalog # M00084-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: PTGS2 (Prostaglandin G/H synthase 2).
- Antibody format: Monoclonal; clone AFHD-16; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PTGS2 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. . Reported cellular localization context: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Tissue expression notes (as provided): Rod shaped photoreceptor cells which mediates vision in dim light.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Cholesterol Metabolism,Endocrine Metabolism,Heart Disease,Hormone Biosynthesis,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipid Signaling,Lipids/Lipoproteins,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Platelets,Signal Transduction,Signaling Pathway,Tumor Biomarkers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PTGS2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PTGS2 expression by Western blot in cell or tissue lysates, Detect PTGS2 in FFPE tissue sections by immunohistochemistry, Enrich PTGS2 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 46 kDa; calculated MW: 68996 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 46 kDa
- Cellular localization (provided): Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
- Tissue details (provided): Rod shaped photoreceptor cells which mediates vision in dim light.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
