| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome c oxidase subunit 4 isoform 2, mitochondrial; Cytochrome c oxidase subunit IV isoform 2; COX IV-2; COX4I2; COX4L2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human COX4I2, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat COX4I2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cytochrome c oxidase subunit 4 isoform 2, mitochondrial (gene COX4I2) in human samples and is validated for Western blot. The predicted molecular weight is 20.0 kDa, and the supplier reports an observed band at about 20 kDa.
COX4I2 is a tissue-restricted isoform of cytochrome c oxidase subunit 4, expressed mainly in lung and some other tissues. It can replace COX4I1 in complex IV, the last enzyme of the respiratory chain, and its expression is increased under low oxygen, so it is studied in oxygen sensing and mitochondrial adaptation.
| Target | Cytochrome c oxidase subunit 4 isoform 2, mitochondrial (gene COX4I2; UniProt Q96KJ9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 20.0 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human COX4I2. Sequence identity with the mouse and rat orthologs: 88.2% and 82.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cytochrome c oxidase subunit 4 isoform 2, mitochondrial in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Highly expressed in lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Liu et al. (2018) MiR-21 regulates the apoptosis of keloid fibroblasts by caspase-8 and the mitochondria-mediated apoptotic signaling pathway via targeting FasL. Biochemistry and Cell Biology. 10.1139/bcb-2017-0306
