| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DNA-binding protein inhibitor ID-1;Class B basic helix-loop-helix protein 24;bHLHb24;Inhibitor of DNA binding 1;Inhibitor of differentiation 1;ID1;BHLHB24, ID; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human CXCL5 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CXCL5 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25C20; isotype IgG; reactivity: Human. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-CXCL5 Rabbit Monoclonal Antibody catalog # M00946. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CXCL5 (DNA-binding protein inhibitor ID-1).
- Antibody format: Monoclonal; clone 25C20; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CXCL5 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity). . Reported cellular localization context: Cytoplasm . Nucleus. Tissue expression notes (as provided): Expressed in the excretory system, thyroid gland and Wilms tumors.
Research relevance and current trends
- Research context keywords from the source record include: Angiogenesis,Cancer,Cancer Susceptibility,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Invasion/Microenvironment,Proto-Oncogenes,Signal Transduction,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate CXCL5 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CXCL5 expression by Western blot in cell or tissue lysates, Localize CXCL5 by immunofluorescence/immunocytochemistry in cultured cells, Enrich CXCL5 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 10 kDa; calculated MW: 16133 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 10 kDa
- Cellular localization (provided): Cytoplasm . Nucleus.
- Tissue details (provided): Expressed in the excretory system, thyroid gland and Wilms tumors.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
