| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C motif chemokine 5; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5; CXCL5; ENA78; SCYB5 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CXCL5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25C20 is a rabbit monoclonal antibody that recognizes C-X-C motif chemokine 5 (gene CXCL5) in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 12.0 kDa, and the supplier reports an observed band at about 8 kDa.
CXCL5 (ENA-78) is a CXC chemokine produced by epithelial cells and other cell types during inflammation. It recruits and activates neutrophils through CXCR2 and has angiogenic activity. It is studied in lung and gut inflammation and in tumor biology.
| Target | C-X-C motif chemokine 5 (gene CXCL5; UniProt P42830, human) |
|---|---|
| Clone | 25C20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 12.0 kDa |
| Observed MW | 8 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CXCL5.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes C-X-C motif chemokine 5 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haichuan Liu et al. (2023) Necroptosis-Related Prognostic Model for Pancreatic Carcinoma Reveals Its Invasion and Metastasis Potential through Hybrid EMT and Immune Escape. Biomedicines. 10.3390/biomedicines11061738
