| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-A2;Cyclin-A;Ccna2;Ccna, Cyca; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant bovine cyclin A. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CY-A1 is a mouse monoclonal antibody that recognizes cyclin-A2 (gene Ccna2) in bovine, human and mouse samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 47.3 kDa, and the supplier reports an observed band at about 55 kDa.
Cyclin A2 (CCNA2) is an essential cell cycle regulator that activates CDK2 during S phase and CDK1 at the G2/M transition. It helps drive DNA replication and entry into mitosis, and because its levels track cell proliferation, it is a common proliferation marker in cell cycle and cancer research.
| Target | Cyclin-A2 (gene Ccna2; UniProt P51943, mouse) |
|---|---|
| Clone | CY-A1 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Bovine, Human, Mouse |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 47.3 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunocytochemistry / Immunofluorescence | 1 µg/mL (Human, Bovine, Mouse) |
|---|---|
| Western blot | 0.5–1 µg/mL (Human, Bovine, Mouse) |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells, SMMC-7721 cells.
Immunogen
Recombinant bovine cyclin A.
Reactivity Notes
The supplier lists reactivity with bovine, human and mouse. UniProt places cyclin-A2 in the nucleus and cytoplasm. Tissue expression noted by UniProt (mouse): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ma Cui et al. (2022) Ubiquitinated AIF is a major mediator of hypoxia-induced mitochondrial dysfunction and pulmonary artery smooth muscle cell proliferation. Cell and Bioscience. 10.1186/s13578-022-00744-3
Min Huang et al. (2022) Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly. Journal of Cellular Physiology. 10.1002/jcp.30693
Zhang Hongyue et al. (2018) Long noncoding RNA Hoxaas3 contributes to hypoxia-induced pulmonary artery smooth muscle cell proliferation. Cardiovascular Research. 10.1093/cvr/cvy250
Songyue Li et al. (2025) Super-Enhancer Target Gene CBP/p300-Interacting Transactivator With Glu/Asp-Rich C-Terminal Domain, 2 Cooperates With Transcription Factor Forkhead Box J3 to Inhibit Pulmonary Vascular Remodeling. Cell Proliferation. 10.1111/cpr.13817
Zhang Chen et al. (2019) MiR-449a-5p mediates mitochondrial dysfunction and phenotypic transition by targeting Myc in pulmonary arterial smooth muscle cells. Journal of Molecular Medicine-Jmm. 10.1007/s00109-019-01751-7
Ma Cui et al. (2017) MiR-125a regulates mitochondrial homeostasis through targeting mitofusin 1 to control hypoxic pulmonary vascular remodeling. Journal of Molecular Medicine-Jmm. 10.1007/s00109-017-1541-5
Chen Lin et al. (2014) Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells. Cell Communication and Signaling. 10.1186/1478-811X-12-18
Yu-Dong Wei et al. (2021) Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells. Zoological Research. 10.24272/j.issn.2095-8137.2020.186
Peiling Wang et al. (2019) Haspin inhibition delays cell cycle progression through interphase in cancer cells. Journal of Cellular Physiology. 10.1002/jcp.29328
Cui Ma et al. (2020) piRNA-63076 contributes to pulmonary arterial smooth muscle cell proliferation through acyl-CoA dehydrogenase. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.15179
