| Field | Specification |
|---|---|
| Target | |
| Alternative names | G1/S-specific cyclin-D2;Vin-1 proto-oncogene;Ccnd2;Vin-1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant human cyclin D2 protein. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DCS-3 is a mouse monoclonal antibody that recognizes G1/S-specific cyclin-D2 (gene Ccnd2) in human and mouse samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 32.8 kDa, and the supplier reports an observed band at about 55 kDa.
Cyclin D2 partners with CDK4 or CDK6 to phosphorylate retinoblastoma family proteins in early G1, releasing E2F and allowing cells to move toward S phase. D-type cyclins respond to growth factor signaling, and cyclin D2 is especially important in lymphocytes, pancreatic beta cells and neural progenitors.
| Target | G1/S-specific cyclin-D2 (gene Ccnd2; UniProt Q04827, rat) |
|---|---|
| Clone | DCS-3 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 32.8 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunocytochemistry / Immunofluorescence | 1 µg/mL (Human, Mouse) |
|---|---|
| Western blot | 2–4 µg/mL (Human, Mouse) |
Immunogen
Recombinant human cyclin D2 protein.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places G1/S-specific cyclin-D2 in the nucleus, cytoplasm and nuclear membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fang Xia et al. (2016) CD274 promotes cell cycle entry of leukemia-initiating cells through JNK/Cyclin D2 signaling. Journal of Hematology & Oncology. 10.1186/s13045-016-0350-6
Chen Feng et al. (2024) Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability. Cardiovascular Toxicology. 10.1007/s12012-024-09865-2
Ruitong Han et al. (2019) piRNA-DQ722010 contributes to prostate hyperplasia of the male offspring mice after the maternal exposed to microcystin-leucine arginine. Prostate. 10.1002/pros.23786
Shuping Ge et al. (2015) An in vitro study on the biocompatibility of WE magnesium alloys. Journal of Biomedical Materials Research Part B-Applied Biomaterials. 10.1002/jbm.b.33388
Xue Yang et al. (2015) Reduced expression of microRNA‑206 regulates cell proliferation via cyclinD2 in gliomas. Molecular Medicine Reports. 10.3892/mmr.2015.3171
