| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | G1/S-specific cyclin-D2;Vin-1 proto-oncogene;Ccnd2;Vin-1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant human cyclin D2 protein. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3) is an antibody targeting CCND2. Common applications include WB, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: DCS-3; isotype: Mouse IgG2a; reactivity: Human,Mouse; observed MW: 55 kDa; calculated MW: 32826 MW.
Boster Bio Anti-Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3) catalog # MA1034. Tested in ICC, WB applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: CCND2 — G1/S-specific cyclin-D2
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: DCS-3; Isotype: Mouse IgG2a
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 55 kDa; Calculated: 32826 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). .
Scientific background (datasheet): Inaba et al. (1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2 (CCND2) gene ia assigned to 12p13.
Cellular localization (datasheet): Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .
Sequence similarities (datasheet): Belongs to the cyclin family. Cyclin D subfamily.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cell Biology,Cell Cycle,Epigenetics and Nuclear Signaling.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
