| Field | Specification |
|---|---|
| Target | |
| Alternative names | 1,25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial;24-OHase;Vitamin D (3) 24-hydroxylase;1.14.13.126;Cytochrome P450 24A1;Cytochrome P450-CC24;CYP24A1;CYP24; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CYP24A1 recombinant protein (Position: E153-R514). Human CYP24A1 shares 86.2% amino acid (aa) sequence identity with both mouse and rat CYP24A1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (gene CYP24A1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 58.9 kDa, and the supplier reports an observed band at about 59 kDa.
CYP24A1 (vitamin D 24-hydroxylase) is a mitochondrial cytochrome P450 that inactivates vitamin D. It hydroxylates both calcidiol and the active hormone calcitriol, starting their breakdown to excretable products. Its expression is strongly induced by calcitriol as a feedback control, and it is studied in calcium and phosphate homeostasis, kidney disease and hypercalcemia.
| Target | 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (gene CYP24A1; UniProt Q07973, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 58.9 kDa |
| Observed MW | 59 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat kidney tissue, mouse kidney tissue, human pancreatic cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CYP24A1 fragment (Glu153–Arg514), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 86.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial in the mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kurosaki Yoshifumi et al. (2024) SARS-CoV-2 infection causes a decline in renal megalin expression and affects vitamin D metabolism in the kidney of K18-hACE2 mice. Scientific Reports. 10.1038/s41598-024-75338-9
