| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 1,25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial;24-OHase;Vitamin D (3) 24-hydroxylase;1.14.13.126;Cytochrome P450 24A1;Cytochrome P450-CC24;CYP24A1;CYP24; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CYP24A1 recombinant protein (Position: E153-R514). Human CYP24A1 shares 86.2% amino acid (aa) sequence identity with both mouse and rat CYP24A1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CYP24A1 (1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CYP24A1 Antibody Picoband® catalog # PB9547. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CYP24A1 recombinant protein (Position: E153-R514). Human CYP24A1 shares 86.2% amino acid (aa) sequence identity with both mouse and rat CYP24A1. (reported region: E153-R514).
- Molecular weight context: reported MW: 59 kDa; calculated MW: 59 kDa
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. And this mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Functional note: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D (3)) and calcitriol (1-alpha,25-dihydroxyvitamin D (3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D (3)-26,23-lactone as end product. Reported localization: Mitochondrion. Expression/tissue context: Expressed in many tissues. .
Research relevance and current trends
- Cancer: Researchers commonly examine how CYP24A1 (1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how CYP24A1 (1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how CYP24A1 (1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CYP24A1 (1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CYP24A1 (1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the cytochrome P450 family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
