| Field | Specification |
|---|---|
| Target | |
| Alternative names | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;1.14.13.13;25-OHD-1 alpha-hydroxylase;25-hydroxyvitamin D (3) 1-alpha-hydroxylase;VD3 1A hydroxylase;Calcidiol 1-monooxygenase;Cytochrome P450 subfamily XXVIIB polypeptide 1;Cytochrome P450C1 alpha;Cytochrome P450VD1-alpha;Cytochrome p450 27B1;CYP27B1;CYP1ALPHA, CYP27B; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1, different from the related mouse and rat sequences by six amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (gene CYP27B1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 56.5 kDa, and the supplier reports an observed band at about 57 kDa.
CYP27B1 (25-hydroxyvitamin D 1α-hydroxylase) is a mitochondrial cytochrome P450, mainly in the kidney, that turns calcidiol into calcitriol, the active vitamin D hormone. This rate-limiting step is tightly controlled by parathyroid hormone, FGF23 and calcium. CYP27B1 is also expressed in immune cells and is studied in calcium and phosphate homeostasis and bone health.
| Target | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (gene CYP27B1; UniProt O15528, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 56.5 kDa |
| Observed MW | 57 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HeLa cells, human Caco-2 cells, rat heart tissue, rat brain tissue, mouse heart tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat kidney tissue, human kidney cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human CYP27B1. It differs from the mouse and rat sequences at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial in the mitochondrion membrane. Tissue expression noted by UniProt (human): Kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tourkova Irina L et al. (2017) Adrenocorticotropic hormone and 1,25-dihydroxyvitamin D3 enhance human osteogenesis in vitro by synergistically accelerating the expression of bone-specific genes. Laboratory Investigation. 10.1038/labinvest.2017.62
