| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;1.14.13.13;25-OHD-1 alpha-hydroxylase;25-hydroxyvitamin D (3) 1-alpha-hydroxylase;VD3 1A hydroxylase;Calcidiol 1-monooxygenase;Cytochrome P450 subfamily XXVIIB polypeptide 1;Cytochrome P450C1 alpha;Cytochrome P450VD1-alpha;Cytochrome p450 27B1;CYP27B1;CYP1ALPHA, CYP27B; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1, different from the related mouse and rat sequences by six amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CYP27B1 (25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CYP27B1 Antibody Picoband® catalog # PB9548. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1, different from the related mouse and rat sequences by six amino acids.
- Molecular weight context: reported MW: 57 kDa; calculated MW: 56504 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial. CYP27B1 belongs to the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. Functional note: Catalyzes the conversion of 25-hydroxyvitamin D3 (25 (OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25 (OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation. Reported localization: Mitochondrion membrane. Expression/tissue context: Kidney.
Research relevance and current trends
- Cancer: Researchers commonly examine how CYP27B1 (25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how CYP27B1 (25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how CYP27B1 (25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CYP27B1 (25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CYP27B1 (25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the cytochrome P450 family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
