| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome P450 2E1;1.14.13.-;4-nitrophenol 2-hydroxylase;1.14.13.n7;CYPIIE1;Cytochrome P450-J;Cytochrome P450 2E1, N-terminally processed;CYP2E1;CYP2E; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CYP2E1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AADC-3 is a rabbit monoclonal antibody that recognizes cytochrome P450 2E1 (gene CYP2E1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 56.8 kDa, and the supplier reports an observed band at about 57 kDa.
CYP2E1 is a liver cytochrome P450 enzyme that oxidizes small molecules such as ethanol, acetone and acetaminophen, as well as fatty acids. It is induced by alcohol and fasting and can produce reactive oxygen species and toxic metabolites, including the acetaminophen metabolite NAPQI. It is studied in alcoholic liver injury, drug toxicity and oxidative stress.
| Target | Cytochrome P450 2E1 (gene CYP2E1; UniProt P05181, human) |
|---|---|
| Clone | AADC-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.8 kDa |
| Observed MW | 57 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human liver cancer tissu.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human CYP2E1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytochrome P450 2E1 in the endoplasmic reticulum membrane, microsome membrane and mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhi-Na Ma et al. (2017) Nephroprotective Effects of Saponins from Leaves of Panax quinquefolius against Cisplatin-Induced Acute Kidney Injury. International Journal of Molecular Sciences. 10.3390/ijms18071407
