| Field | Specification |
|---|---|
| Target | |
| Alternative names | Steroid 17-alpha-hydroxylase/17,20 lyase;1.14.14.19 ;17-alpha-hydroxyprogesterone aldolase;4.1.2.30 ;CYPXVII;Cytochrome P450 17A1;Cytochrome P450-C17;Cytochrome P450c17;Steroid 17-alpha-monooxygenase;CYP17A1;CYP17, S17AH; |
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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CYP17A1, different from the related mouse and rat sequences by ten amino acids. |
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Overview
This antibody is intended for detection of CYP17A1 (Steroid 17-alpha-hydroxylase/17,20 lyase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cytochrome P450 17A1/CYP17A1 Antibody Picoband® catalog # A00615-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP17A1, different from the related mouse and rat sequences by ten amino acids.
- Molecular weight context: reported MW: 57 kDa; calculated MW: 57371 MW
- Reactivity: Human,Rat
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Steroid 17-alpha-hydroxylase/17,20 lyase; Steroid 17-alpha-hydroxylase/17,20 lyase. Cytochrome P450 17A1, also called steroid 17α-monooxygenase, is an enzyme of the hydroxylase type that in humans is encoded by the CYP17A1 gene on chromosome 10. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. Functional note: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. . Reported localization: Membrane . Expression/tissue context: Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. .
Research relevance and current trends
- Cancer: Researchers commonly examine how CYP17A1 (Steroid 17-alpha-hydroxylase/17,20 lyase) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how CYP17A1 (Steroid 17-alpha-hydroxylase/17,20 lyase) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how CYP17A1 (Steroid 17-alpha-hydroxylase/17,20 lyase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CYP17A1 (Steroid 17-alpha-hydroxylase/17,20 lyase) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CYP17A1 (Steroid 17-alpha-hydroxylase/17,20 lyase) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
