| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome P450 1A1;1.14.14.1;CYPIA1;Cytochrome P450 form 6;Cytochrome P450-C;Cytochrome P450-P1;CYP1A1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cytochrome P450 1A1 (gene CYP1A1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 58.2 kDa, and the supplier reports an observed band at about 58 kDa.
CYP1A1 is a cytochrome P450 enzyme mainly expressed outside the liver and strongly induced through the aryl hydrocarbon receptor (AHR) by polycyclic aromatic hydrocarbons and dioxins. It oxidizes these compounds, which can generate reactive, carcinogenic intermediates, and it also hydroxylates estrogens and fatty acids. It is a classic marker of AHR activation in toxicology.
| Target | Cytochrome P450 1A1 (gene CYP1A1; UniProt P04798, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 58.2 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human, Mouse) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, rat liver tissue, rat RH-35 cells, rat PC-12 cells, mouse liver tissue, mouse HEPA1/6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat kidney tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): mouse kidney tissue, human placenta tissue.
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells, HeLa cells, K562 cells.
Immunogen
Recombinant human CYP1A1 fragment (His183–Asp320), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 81.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytochrome P450 1A1 in the endoplasmic reticulum membrane, mitochondrion inner membrane, microsome membrane and cytoplasm. Tissue expression noted by UniProt (human): Lung, lymphocytes and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Siyi Wang et al. (2024) Iron-doped diesel exhaust early-in-life inhalation-induced cardiopulmonary toxicity in chicken embryo: Roles of ferroptosis and acyl hydrocarbon signaling. Environmental Pollution. 10.1016/j.envpol.2024.125085
Caihong Wang et al. (2024) Kynurenine Attenuates Ulcerative Colitis Mediated by the Aryl Hydrocarbon Receptor. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.4c04933
Huancheng Xie et al. (2026) Stabilization of MHCII on dendritic cells via AhR-mediated blockade of proteasomal degradation drives Th17 responses to exogenous antigens. International Immunopharmacology. 10.1016/j.intimp.2026.117011
Jianchao Li et al. (2025) Niuhuang Jiedu Tablet's compatibility alleviates realgar-induced intestinal toxicity by activating aryl hydrocarbon receptor pathway via Akkermansia and indole−3−lactate. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120260
Gaber Marwa et al. (2021) Effect of polyaromatic hydrocarbons on cellular cytochrome P450 1A induction. Ocean and Coastal Research. 10.1590/2675-2824069.21026mg
