| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome P450 1A2;1.14.14.1;CYPIA2;Cytochrome P (3)450;Cytochrome P450 4;Cytochrome P450-P3;CYP1A2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CYP1A2 recombinant protein (Position: H185-D320). Human CYP1A2 shares 69.9% and 72.1% amino acid (aa) sequence identity with mouse and rat CYP1A2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cytochrome P450 1A2 (gene CYP1A2) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 58.4 kDa, and the supplier reports an observed band at about 58 kDa.
CYP1A2 is a liver cytochrome P450 enzyme that metabolizes many drugs and chemicals, including caffeine, theophylline and some aromatic amines, and it also acts on estrogens and fatty acids. Its expression is induced through the aryl hydrocarbon receptor, for example by smoking, so it is studied in drug metabolism, drug interactions and toxicology.
| Target | Cytochrome P450 1A2 (gene CYP1A2; UniProt P05177, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 58.4 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human hccp tissue, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue, rat liver tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CYP1A2 fragment (His185–Asp320), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 69.9% and 72.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytochrome P450 1A2 in the endoplasmic reticulum membrane and microsome membrane. Tissue expression noted by UniProt (human): Liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuping Zhou et al. (2023) Eight Zhes Decoction ameliorates the lipid dysfunction of nonalcoholic fatty liver disease using integrated lipidomics, network pharmacology and pharmacokinetics. Journal of Pharmaceutical Analysis. 10.1016/j.jpha.2023.05.012
Jiang Yiming et al. (2014) Schisandrol B Protects Against Acetaminophen-Induced Hepatotoxicity by Inhibition of CYP-Mediated Bioactivation and Regulation of Liver Regeneration. Toxicological Sciences. 10.1093/toxsci/kfu216
Sijing Dong et al. (2019) Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sciences. 10.1016/j.lfs.2019.04.019
Xiaomei Fan et al. (2014) Wuzhi Tablet (Schisandra Sphenanthera Extract) Protects against Acetaminophen-Induced Hepatotoxicity by Inhibition of CYP-Mediated Bioactivation and Regulation of NRF2-ARE and p53/p21 Pathways. Drug Metabolism and Disposition. 10.1124/dmd.114.059535
Yinsheng Li et al. (2017) Effect of enrofloxacin and roxarsone on CYP450s in pig. Research in Veterinary Science. 10.1016/j.rvsc.2017.11.014
Dong Sijing et al. (2018) Protective effects and possible molecular mechanism of Hovenia dulcis Thunb. extract on acetaminophen-induced hepatotoxicity. Pharmazie. 10.1691/ph.2018.8650
