| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytoglobin;Histoglobin;HGb;Stellate cell activation-associated protein;CYGB;STAP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Cytoglobin recombinant protein (Position: M1-P190). Human Cytoglobin shares 95.3% and 93.7% amino acid (aa) sequence identity with mouse and rat Cytoglobin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets cytoglobin (gene CYGB) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 21.4 kDa, and the supplier reports an observed band at about 21 kDa.
Cytoglobin (CYGB) is a vertebrate globin with a hexacoordinated heme, expressed mainly in fibroblast-like cells such as hepatic stellate cells and in some neurons. It is thought to consume nitric oxide and protect cells from oxidative stress, which could influence vascular tone. Its physiological roles are still being defined, and it is studied in liver fibrosis and hypoxia biology.
| Target | Cytoglobin (gene CYGB; UniProt Q8WWM9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 21.4 kDa |
| Observed MW | 21 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
Samples with a confirmed band (WB): rat small intestine tissue, mouse small intestine tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, mouse cardiac muscle tissue, rat intestine tissue, human intestinal cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human Cytoglobin fragment (Met1–Pro190), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95.3% and 93.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytoglobin in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
