| Field | Specification |
|---|---|
| Target | |
| Alternative names | Diacylglycerol O-acyltransferase 1;2.3.1.20 ;ACAT-related gene product 1;Acyl-CoA retinol O-fatty-acyltransferase;ARAT;Retinol O-fatty-acyltransferase;2.3.1.76 ;Diglyceride acyltransferase;DGAT1;AGRP1, DGAT; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human DGAT1, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets diacylglycerol O-acyltransferase 1 (gene DGAT1) in human and rat samples and is validated for Western blot. The predicted molecular weight is 55.3 kDa, and the supplier reports an observed band at about 60 kDa.
DGAT1 (diacylglycerol O-acyltransferase 1) is an endoplasmic reticulum enzyme that attaches a fatty acyl-CoA to diacylglycerol, the final committed step of triglyceride synthesis. It is highly active in the small intestine, where it supports absorption of dietary fat, as well as in liver and adipose tissue. DGAT1 is studied in lipid metabolism, obesity and metabolic disease.
| Target | Diacylglycerol O-acyltransferase 1 (gene DGAT1; UniProt O75907, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 55.3 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat kidney tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human DGAT1. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places diacylglycerol O-acyltransferase 1 in the endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuping Zhou et al. (2023) Eight Zhes Decoction ameliorates the lipid dysfunction of nonalcoholic fatty liver disease using integrated lipidomics, network pharmacology and pharmacokinetics. Journal of Pharmaceutical Analysis. 10.1016/j.jpha.2023.05.012
Lingling Zhang et al. (2022) mTORC2 Facilitates Liver Regeneration Through Sphingolipid-Induced PPAR-α-Fatty Acid Oxidation. Cellular and Molecular Gastroenterology and Hepatology. 10.1016/j.jcmgh.2022.07.011
Ting Chen et al. (2026) Multi-omics integration reveals the ameliorative effects and underlying mechanisms of Astragalus membranaceus (Huangqi)-Fuzhuan brick tea on nonalcoholic fatty liver disease. Journal of Ethnopharmacology. 10.1016/j.jep.2026.121616
