| Field | Specification |
|---|---|
| Target | |
| Alternative names | Drebrin;Developmentally-regulated brain protein;DBN1;D0S117E; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Drebrin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABDC-4 is a rabbit monoclonal antibody that recognizes drebrin (gene DBN1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 71.4 kDa, and the supplier reports an observed band at about 120 kDa.
Drebrin (DBN1) is an actin-binding protein that stabilizes and organizes F-actin in cell protrusions. In neurons it is concentrated in dendritic spines, where it shapes spine structure and supports synaptic plasticity, and it also takes part in actin remodeling at the immunological synapse. Drebrin is studied in neuronal development, synapse biology and cell migration.
| Target | Drebrin (gene DBN1; UniProt Q16643, human) |
|---|---|
| Clone | ABDC-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 71.4 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human drebrin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places drebrin in the cytoplasm, cell projection and cell junction. Tissue expression noted by UniProt (human): Expressed in the brain, with expression in the molecular layer of the dentate gyrus, stratum pyramidale, and stratum radiatum of the hippocampus (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Rafaela Pedro Silva et al. (2025) Phosphoprofile reorganization of the actin binding protein Drebrin during long term depression. Frontiers in Molecular Neuroscience. 10.3389/fnmol.2025.1697642
