| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dynein axonemal intermediate chain 1; Axonemal dynein intermediate chain 1; DNAI1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Dynein intermediate chain 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17D80 is a rabbit monoclonal antibody that recognizes dynein axonemal intermediate chain 1 (gene DNAI1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 79.3 kDa, and the supplier reports an observed band at about 79 kDa.
DNAI1 is an intermediate chain of the outer dynein arms that power the beating of motile cilia and sperm flagella. It helps attach the dynein motors to the axoneme. Mutations in DNAI1 cause primary ciliary dyskinesia, so it is studied in ciliary structure and function in the airway and in reproduction.
| Target | Dynein axonemal intermediate chain 1 (gene DNAI1; UniProt Q9UI46, human) |
|---|---|
| Clone | 17D80 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 79.3 kDa |
| Observed MW | 79 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): mouse testis.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human dynein intermediate chain 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dynein axonemal intermediate chain 1 in the dynein axonemal particle, cytoplasm and cell projection. Tissue expression noted by UniProt (human): Expressed in respiratory ciliated cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
