| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dynein light chain 1, cytoplasmic;8 kDa dynein light chain;DLC8;Dynein light chain LC8-type 1;Protein inhibitor of neuronal nitric oxide synthase;PIN;DYNLL1;DLC1, DNCL1, DNCLC1, HDLC1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DYNLL1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EDA-4 is a rabbit monoclonal antibody that recognizes dynein light chain 1, cytoplasmic (gene DYNLL1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 10.4 kDa, and the supplier reports an observed band at about 12 kDa.
DYNLL1 (also called LC8 or PIN) is a small light chain of cytoplasmic dynein 1, the main minus-end-directed microtubule motor. Beyond dynein, it binds many other proteins through a short linear motif and acts as a dimerization hub, so it is studied in intracellular transport and protein complex assembly.
| Target | Dynein light chain 1, cytoplasmic (gene DYNLL1; UniProt P63167, human) |
|---|---|
| Clone | EDA-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 10.4 kDa |
| Observed MW | 12 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells, human SIHA cells, human A549 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human bladder cancer tissue, human lung cancer tissue, human liver cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human breast cancer tissue, human tonsil tissue, human renal cancer tissue, human cervical cancer tissue, human ovarian cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed result (IP): A549 cells.
Immunogen
Synthetic peptide based on the sequence of human DYNLL1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dynein light chain 1, cytoplasmic in the chromosome, cytoplasm, nucleus and mitochondrion. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
