| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dynein light chain 1, cytoplasmic;8 kDa dynein light chain;DLC8;Dynein light chain LC8-type 1;Protein inhibitor of neuronal nitric oxide synthase;PIN;DYNLL1;DLC1, DNCL1, DNCLC1, HDLC1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human DYNLL1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-DYNLL1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone EDA-4; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-DYNLL1/Pin Rabbit Monoclonal Antibody catalog # M03454. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: DYNLL1 (Dynein light chain 1, cytoplasmic).
- Antibody format: Monoclonal; clone EDA-4; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
DYNLL1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Reported cellular localization context: Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Upon induction of apoptosis translocates together with BCL2L11 to mitochondria. Tissue expression notes (as provided): Ubiquitous. .
Research relevance and current trends
- Research context keywords from the source record include: Apoptosis,Apoptotic Markers,Cancer,Cell Biology,Cell Death,Cytoskeleton,Cytoskeleton/ECM,DNA/RNA,DNA Damage & Repair,DNA Damage Response,Dynein,Epigenetics and Nuclear Signaling,Intracellular,Invasion/Microenvironment,Metabolism,Metabolism Processes,Motor Proteins,Neuroscience,Neurotransmission,Oncoproteins/Suppressors,p53 Pathway,Pathways and Processes,Signal Transduction,Tumor Suppressors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate DYNLL1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DYNLL1 expression by Western blot in cell or tissue lysates, Detect DYNLL1 in FFPE tissue sections by immunohistochemistry, Localize DYNLL1 by immunofluorescence/immunocytochemistry in cultured cells, Enrich DYNLL1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 32 kDa; calculated MW: 10366 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 32 kDa
- Cellular localization (provided): Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Upon induction of apoptosis translocates together with BCL2L11 to mitochondria.
- Tissue details (provided): Ubiquitous. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
