| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Angiotensin-converting enzyme; ACE; Dipeptidyl carboxypeptidase I; Kininase II; CD143; Angiotensin-converting enzyme, soluble form; Ace; Dcp1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human E2F1 recombinant protein (Position: K89-A328). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-E2F1 Antibody Picoband® is an antibody for E2F1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: E2F1 (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1); UniProt: Q01094
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 70 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-E2F1 Antibody Picoband® catalog # A00257-2.
Biological background
Biological context: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
Expression and localization notes: cellular localization: Angiotensin-converting enzyme, soluble form: Secreted., tissue context: Testis-specific isoform is expressed in spermatocytes, adult testis..
Common research applications
- Western blotting (WB): Compare E2F1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of E2F1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify E2F1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product(pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein(pRB).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Angiotensin-converting enzyme, soluble form: Secreted.
- Tissue details: Testis-specific isoform is expressed in spermatocytes, adult testis.
- Research category: Cancer,Cancer Metabolism,Cardiovascular,Cell Biology,Cofactors, Vitamins/Minerals,Hematopoietic Progenitors,Metabolism,Metabolism Processes,Metalloprotease,Pathways and Processes,Proteolysis/Ubiquitin,Proteolytic Enzymes,Response To Hypoxia,Serum Proteins,Signal Transduction,Stem Cells,Surface Molecules,Vasculature,Vitamins/Minerals
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
