| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endothelin-converting enzyme 1;ECE-1;3.4.24.71;ECE1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ECE1 recombinant protein (Position: M18-T233). Human ECE1 shares 94% and 93.5% amino acid (aa) sequence identity with mouse and rat ECE1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets endothelin-converting enzyme 1 (gene ECE1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 87.2 kDa, and the supplier reports an observed band at about 120–130 kDa.
Endothelin-converting enzyme 1 (ECE1) is a membrane-bound zinc metalloprotease that cleaves inactive big endothelin-1 to release the potent vasoconstrictor endothelin-1. It is studied in vascular tone, cardiovascular development and endothelin signaling.
| Target | Endothelin-converting enzyme 1 (gene ECE1; UniProt P42892, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 87.2 kDa |
| Observed MW | 120–130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human HUVEC cells, human MCF-7 cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ECE1 fragment (Met18–Thr233), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94% and 93.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endothelin-converting enzyme 1 in the cell membrane. Tissue expression noted by UniProt (human): All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Bingqian et al. (2014) Endoplasmic reticulum stress promotes amyloid-beta peptides production in RGC-5 cells. Cell Stress & Chaperones. 10.1007/s12192-014-0506-7
