| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cytoplasmic aconitate hydratase;Aconitase;4.2.1.3;Citrate hydro-lyase;Ferritin repressor protein;Iron regulatory protein 1;IRP1;Iron-responsive element-binding protein 1;IRE-BP 1;ACO1;IREB1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human EDG2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-LPAR1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ACGE-12; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-EDG2 LPAR1 Rabbit Monoclonal Antibody catalog # M02782. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: LPAR1 (Cytoplasmic aconitate hydratase).
- Antibody format: Monoclonal; clone ACGE-12; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
LPAR1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. . Reported cellular localization context: Cytoplasm. Tissue expression notes (as provided): Widely expressed.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cancer,Cardiovascular,Cofactors, Vitamins/Minerals,G Protein Signaling,Heart Disease,Lipid Signaling,Metabolism,NFKB Pathway,Nuclear Signaling,Pathways and Processes,Signal Transduction,Signaling Pathway,Vascular Inflammation,Vitamins/Minerals.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate LPAR1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect LPAR1 expression by Western blot in cell or tissue lysates, Detect LPAR1 in FFPE tissue sections by immunohistochemistry, Enrich LPAR1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 70 kDa, 90 kDa; calculated MW: 98399 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 70 kDa, 90 kDa
- Cellular localization (provided): Cytoplasm.
- Tissue details (provided): Widely expressed.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
