| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysophosphatidic acid receptor 1; LPA receptor 1; LPA-1; Lysophosphatidic acid receptor Edg-2; LPAR1; EDG2; LPA1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human EDG2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACGE-12 is a rabbit monoclonal antibody that recognizes lysophosphatidic acid receptor 1 (gene LPAR1) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 41.1 kDa, and the supplier reports an observed band at about 39 kDa.
LPA1 (LPAR1, EDG2) is a G protein-coupled receptor for lysophosphatidic acid, a bioactive lipid. It couples to Gi/o, Gq and G12/13 to regulate cytoskeletal reorganization, cell migration, proliferation and survival. LPA1 is studied in nervous system development, fibrosis, wound healing and cancer.
| Target | Lysophosphatidic acid receptor 1 (gene LPAR1; UniProt Q92633, human) |
|---|---|
| Clone | ACGE-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.1 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human EDG2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places lysophosphatidic acid receptor 1 in the cell surface, cell membrane and endosome. Tissue expression noted by UniProt (human): Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
