| Field | Specification |
|---|---|
| Target | |
| Alternative names | Epidermal growth factor receptor; Egfr |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human EGFR recombinant protein (Position: D1006-A1210). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects epidermal growth factor receptor (gene EGFR) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 134.3 kDa, and the supplier reports an observed band at about 180 kDa.
Epidermal growth factor receptor (EGFR, also called ErbB1 or HER1) is a receptor tyrosine kinase activated by EGF, TGF-α and related ligands. Ligand binding triggers receptor dimerization and activation of RAS/MAPK, PI3K/AKT and other pathways that drive proliferation and survival, and EGFR is a major target in cancer research.
| Target | Epidermal growth factor receptor (gene EGFR; UniProt P00533, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 134.3 kDa |
| Observed MW | 180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A431 cells, human A549 cells, human U87 cells, human HeLa cells, human A549- WT cells, human A549-EGFR KO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human EGFR fragment (Asp1006–Ala1210), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places epidermal growth factor receptor in the cell membrane, endoplasmic reticulum membrane, Golgi apparatus membrane and nuclear membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dandan Wang et al. (2022) Microbiota, co-metabolites, and network pharmacology reveal the alteration of the ginsenoside fraction on inflammatory bowel disease. Journal of Ginseng Research. 10.1016/j.jgr.2022.04.001
Cui-cui Sun et al. (2011) Inhibition of angiogenesis involves in anticancer activity of riccardin D, a macrocyclic bisbibenzyl, in human lung carcinoma. European Journal of Pharmacology. 10.1016/j.ejphar.2011.06.013
Yan Feng et al. (2007) Epidermal Growth Factor Receptor in Cultured Human Retinal Pigment Epithelial Cells. Ophthalmologica. 10.1159/000101926
