| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endonuclease G, mitochondrial; Endo G; ENDOG |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Endo G |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18E31 is a rabbit monoclonal antibody that recognizes endonuclease G, mitochondrial (gene ENDOG) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 32.6 kDa, and the supplier reports an observed band at about 33 kDa.
Endonuclease G (ENDOG) is a nuclease encoded in the nucleus and located in mitochondria. During apoptosis it can be released and move to the nucleus, where it contributes to DNA fragmentation independently of caspases. It also processes mitochondrial DNA under oxidative stress and has been linked to autophagy regulation, making it a target in cell death research.
| Target | Endonuclease G, mitochondrial (gene ENDOG; UniProt Q14249, human) |
|---|---|
| Clone | 18E31 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 32.6 kDa |
| Observed MW | 33 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human endo G.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endonuclease G, mitochondrial in the mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan-Qing Guan et al. (2011) Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs. Biomaterials. 10.1016/j.biomaterials.2011.01.060
Hong Zhang et al. (2017) RETRACTED: Icariin induces apoptosis in acute promyelocytic leukemia by targeting PIM1. Pharmacological Reports. 10.1016/j.pharep.2017.06.005
