| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;C-myc promoter-binding protein;Enolase 1;MBP-1;MPB-1;Non-neural enolase;NNE;Phosphopyruvate hydratase;Plasminogen-binding protein;ENO1;ENO1L1, MBPB1, MPB1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human ENO1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody is an antibody targeting ENO1. Common applications include WB, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AEA-5; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 47 kDa; calculated MW: 47169 MW.
Boster Bio Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody catalog # M01250. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ENO1 — Alpha-enolase
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AEA-5; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 47 kDa; Calculated: 47169 MW
Biological background
Protein function (datasheet): Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Cellular localization (datasheet): Cytoplasm . Cell membrane . Cytoplasm, myofibril, sarcomere, M line . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Tissue details (datasheet): The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cancer Metabolism,Cancer Susceptibility,Carbohydrate Metabolism,Co-Factors,Cofactors, Vitamins/Minerals,Energy Metabolism,Energy Transfer Pathways,Epigenetics and Nuclear Signaling,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism Of Carbohydrates,Oncoproteins/Suppressors,Pathways and Processes,Signal Transduction,Transcription,Transcription Factors,Tumor Suppressors.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
