| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;C-myc promoter-binding protein;Enolase 1;MBP-1;MPB-1;Non-neural enolase;NNE;Phosphopyruvate hydratase;Plasminogen-binding protein;ENO1;ENO1L1, MBPB1, MPB1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ENO1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEA-5 is a rabbit monoclonal antibody that recognizes alpha-enolase (gene ENO1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 47.2 kDa, and the supplier reports an observed band at about 47 kDa.
Alpha-enolase (ENO1) is the glycolytic enzyme that interconverts 2-phosphoglycerate and phosphoenolpyruvate, and it is expressed in most tissues. It can also appear on the cell surface as a plasminogen-binding receptor, and an alternative product (MBP-1) represses the MYC promoter, so ENO1 is studied in metabolism, cancer and autoimmunity.
| Target | Alpha-enolase (gene ENO1; UniProt P06733, human) |
|---|---|
| Clone | AEA-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.2 kDa |
| Observed MW | 47 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human MCF-7 cells, human HeLa cells, human U251 cells, human A549 cells, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ENO1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alpha-enolase in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
