| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nitric oxide synthase, endothelial; Constitutive NOS; Cnos; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human eNOS/NOS3 recombinant protein (Position: Q1052-P1203). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-eNOS/NOS3 Antibody Picoband® is an antibody for NOS3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: NOS3 (nitric oxide synthase 3 (endothelial cell)); UniProt: P29474
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 133 kDa, calculated 149527 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-eNOS/NOS3 Antibody Picoband® catalog # A01604-2.
Biological background
Biological context: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Expression and localization notes: cellular localization: Cytoskeleton. Cell membrane. Golgi apparatus. Caveola., tissue context: Platelets, placenta, liver and kidney..
Common research applications
- Western blotting (WB): Compare NOS3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of NOS3 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify NOS3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: NOS3 (Nitric Oxide Synthase 3), also called ENOS, a nitric oxide synthase that generates NO in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility tocoronary spasm. Fulton et al. concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression of NOS3 in human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HAOVSMCs) was inversely proportional to that of NOS3AS.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoskeleton. Cell membrane. Golgi apparatus. Caveola.
- Tissue details: Platelets, placenta, liver and kidney.
- Research category: Adapters,Cancer,Cytoplasmic,G Protein Signaling,Regulators,Signal Transduction,Signaling Pathway,Small G Proteins
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
