| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bifunctional epoxide hydrolase 2;Cytosolic epoxide hydrolase 2;CEH;3.3.2.10;Epoxide hydratase;Soluble epoxide hydrolase;SEH;Lipid-phosphate phosphatase;3.1.3.76;EPHX2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human EPHX2, different from the related mouse sequence by seven amino acids, and from the related rat sequence by eight amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of EPHX2 (Bifunctional epoxide hydrolase 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-EPHX2 Antibody Picoband® catalog # A01999-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human EPHX2, different from the related mouse sequence by seven amino acids, and from the related rat sequence by eight amino acids.
- Molecular weight context: reported MW: 63 kDa; calculated MW: 62616 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Bifunctional epoxide hydrolase 2; Bifunctional epoxide hydrolase 2. Soluble epoxide hydrolase (sEH) is a bifunctional enzyme that in humans is encoded by the EPHX2 gene. It is mapped to 8p21.2-p21.1. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. Functional note: Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. . Reported localization: Cytoplasm. Peroxisome. Expression/tissue context: Ubiquitous. A high level expression is seen in secretory tissues.
Research relevance and current trends
- Cancer Metabolism: Researchers commonly examine how EPHX2 (Bifunctional epoxide hydrolase 2) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how EPHX2 (Bifunctional epoxide hydrolase 2) relates to this theme using model systems and orthogonal readouts.
- Cholesterol Metabolism: Researchers commonly examine how EPHX2 (Bifunctional epoxide hydrolase 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative EPHX2 (Bifunctional epoxide hydrolase 2) levels across conditions; band patterns may reflect isoforms and processing.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
