| Field | Specification |
|---|---|
| Target | |
| Alternative names | HER4,Tyrosine kinase-type cell surface receptor HER4,p180erbB4,s80HER4,ERBB4,4ICD,Receptor tyrosine-protein kinase erbB-4,Proto-oncogene-like protein c-ErbB-4,E4ICD |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli - derived recombinant Human ERBB4 (Cys29-Asn278). |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This antibody is intended for research detection of ERBB4.
Target
ERBB4 (Tyrosine kinase-type cell surface receptor HER4)
Target biology
ERBB4 (Tyrosine kinase-type cell surface receptor HER4) is a receptor protein that mediates cellular responses to extracellular cues such as cytokines, growth factors, or environmental ligands. Many receptors signal through kinase cascades and transcriptional regulators that control proliferation, differentiation, or inflammatory programs.
Expected localization
membrane-associated (may vary by cell type and experimental conditions).
Research context
Commonly used in Cell Signaling workflows to study expression changes, pathway state, and cellular localization of ERBB4.
Compatible workflows
- ELISA
- IHC
- WB
Experimental notes
- Western blot: optimize sample preparation (reducing vs. non‑reducing and denaturing conditions) and titrate antibody to balance sensitivity and background. Apparent molecular weight may vary with isoforms and post‑translational modifications (e.g., glycosylation or phosphorylation).
- IHC: epitope accessibility can depend strongly on fixation and antigen retrieval. Test heat‑induced retrieval buffers (citrate pH ~6 or Tris‑EDTA pH ~9) and adjust retrieval time to improve signal-to-background.
- ELISA: performance is sensitive to coating conditions and blocking chemistry; optimize capture density and blocking reagent to reduce non‑specific binding.
Controls & interpretation
Recommended controls include a positive sample reported to express the target (literature‑validated cell line or tissue), a negative/low‑expression sample when available, and ideally genetic knockdown/knockout or peptide‑blocking approaches to confirm specificity.
Immunogen context
E. coli - derived recombinant Human ERBB4 (Cys29-Asn278).
Intended use
For research use only. Not for diagnostic procedures.
ERBB4 (HER4) is a member of the EGFR/ErbB family of receptor tyrosine kinases.
It is a Rabbit polyclonal antibody.
This antibody is validated for ELISA, immunohistochemistry (IHC), and Western blot (WB). Optimal dilutions should be determined by the end user for each application.
Predicted/tested reactivity: human, mouse, and rat. Confirm reactivity for your species and sample type with appropriate controls.
No. This antibody is for in vitro research use only (RUO); it is not intended for diagnostic or therapeutic use in humans or animals.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
