Anti-ERR alpha Rabbit Monoclonal Antibody

SKU:BHA21008425
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Overview
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Anti-ESRRA antibody (Rabbit monoclonal, clone EIO-5, Rabbit IgG). Validated for Western blot, immunoprecipitation and flow cytometry. Reactivity: Human, Mouse, Rat. Recognizes steroid hormone receptor ERR1.
Target ESRRA
clone number EIO-5
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB, IP, Flow
Options selector
Catalog no. Size Conjugation
M03339 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IP,Flow Cytometry
      Application details: WB 1:1000-1:2000<br>IP 1:50<br>FC 1:20
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ESRRA
Alternative names Steroid hormone receptor ERR1;Estrogen receptor-like 1;Estrogen-related receptor alpha;ERR-alpha;Nuclear receptor subfamily 3 group B member 1;ESRRA;ERR1, ESRL1, NR3B1;
Gene ID 2101
UniProt # P11474
Host Rabbit
Clone EIO-5
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunoprecipitation
  • Flow Cytometry
Immunogen A synthesized peptide derived from human ERR alpha
Molecular weight 45.5 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M03339
Main SKU BHA21008425
Rabbit Monoclonal

Product Overview

Clone EIO-5 is a rabbit monoclonal antibody that recognizes steroid hormone receptor ERR1 (gene ESRRA) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 45.5 kDa, and the supplier reports an observed band at about 55 kDa.

Estrogen-related receptor alpha (ESRRA, ERRα) is an orphan nuclear receptor that is active without a known ligand. Together with the coactivator PGC-1α, it drives genes for mitochondrial biogenesis, fatty acid oxidation and oxidative phosphorylation, and it is studied in energy metabolism, muscle and cancer.

Target Steroid hormone receptor ERR1 (gene ESRRA; UniProt P11474, human)
Clone EIO-5
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 45.5 kDa
Observed MW 55 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:1000–1:2000
Immunoprecipitation 1:50
Flow cytometry 1:20

Immunogen

Synthetic peptide based on the sequence of human ERR alpha.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places steroid hormone receptor ERR1 in the nucleus and cytoplasm.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:1000–1:2000; Flow cytometry 1:20; Immunoprecipitation 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.Has this antibody been tested on zebrafish samples?
A.Reactivity with zebrafish samples has not been tested by the supplier. Aligning the immunogen sequence with the zebrafish ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 45.5 kDa; observed band at about 55 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human ERR alpha.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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