| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Homer protein homolog 3;Homer-3;HOMER3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human ETF/TEAD2 recombinant protein (Position: K125-M398). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ETF/TEAD2 Antibody Picoband® is an antibody for TEAD2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: TEAD2 (Homer protein homolog 3); UniProt: Q15562
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 49 kDa, calculated 39836 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-ETF/TEAD2 Antibody Picoband® catalog # A09168-2.
Biological background
Biological context: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
Expression and localization notes: cellular localization: Cytoplasm . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse . Postsynaptic density of neuronal cells. ., tissue context: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas..
Common research applications
- Western blotting (WB): Compare TEAD2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of TEAD2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify TEAD2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: TEAD2 (ETF, ETEF-1, TEF-4), together with TEAD1, defines a novel family of transcription factors, the TEAD family, highly conserved through evolution. TEAD proteins were notably found in Drosophila (Scalloped), C. elegans (egl -44), S. cerevisiae and A. nidulans. TEAD2 has been less studied than TEAD1 but a few studies revealed its role during development. TEAD2 is a member of the mammalian TEAD transcription factor family (initially named the transcriptional enhancer factor (TEF) family), which contain the TEA/ATTS DNA-binding domain.[7] Members of the family in mammals are TEAD1, TEAD2, TEAD3, TEAD4.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse . Postsynaptic density of neuronal cells. .
- Tissue details: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Research category: Receptors / Channels,Synapse Marker
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
