| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ezrin;Cytovillin;Villin-2;p81;EZR;VIL2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Ezrin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAAI-5 is a rabbit monoclonal antibody that recognizes ezrin in human samples and is validated for Western blot and IHC. The predicted molecular weight is 69.4 kDa, and the supplier reports an observed band at about 81 kDa.
Ezrin (EZR) is an ERM-family protein that links the plasma membrane to the actin cytoskeleton. When activated by phosphorylation and PI(4,5)P2 binding, it organizes microvilli and membrane ruffles in epithelial cells and supports cell adhesion, migration and signaling, and it is studied in tumor metastasis.
| Target | Ezrin (gene EZR; UniProt P15311, human) |
|---|---|
| Clone | AAAI-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.4 kDa |
| Observed MW | 81 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ezrin.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ezrin in the apical cell membrane, cell projection and cytoplasm. Tissue expression noted by UniProt (human): Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
De-Kun Wu et al. (2024) A mechanistic study of Anwei decoction intervention in a rat model of gastric intestinal metaplasia through the endoplasmic reticulum stress–autophagy pathway. Tissue & Cell. 10.1016/j.tice.2024.102317
