| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-2-HS-glycoprotein;Countertrypin;Fetuin-A;Ahsg;Fetua; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Fetuin A recombinant protein (Position: A19-I345). Mouse Fetuin A shares 57% and 85% amino acid (aa) sequence identity with human and rat Fetuin A, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects alpha-2-HS-glycoprotein (gene Ahsg) in mouse samples and is validated for Western blot. The predicted molecular weight is 37.3 kDa, and the supplier reports an observed band at about 55–60 kDa.
Fetuin-A (AHSG, alpha-2-HS-glycoprotein) is an abundant plasma protein made mainly by the liver. It binds calcium phosphate and helps prevent unwanted mineral deposition, and it is studied in bone mineralization, vascular calcification, insulin resistance and inflammation.
| Target | Alpha-2-HS-glycoprotein (gene Ahsg; UniProt P29699, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 37.3 kDa |
| Observed MW | 55–60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|
Samples with a confirmed band (WB): mouse plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Fetuin A fragment (Ala19–Ile345), expressed in E. coli. Sequence identity with the human and rat orthologs: 57% and 85%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes alpha-2-HS-glycoprotein as a secreted protein. Tissue expression noted by UniProt (mouse): Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mengru Zhuang et al. (2018) Reelin regulates male mouse reproductive capacity via the sertoli cells. Journal of Cellular Biochemistry. 10.1002/jcb.26824
