| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 21;FGF-21;FGF21;UNQ3115/PRO10196; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human FGF21 recombinant protein (Position: H29-S209). Human FGF21 shares 80.7% amino acid (aa) sequence identity with mouse FGF21. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes fibroblast growth factor 21 (gene FGF21) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 22.3 kDa, and the supplier reports an observed band at about 22 kDa.
Fibroblast growth factor 21 (FGF21) is an endocrine FGF made mainly by the liver in response to fasting and nutritional stress. It signals through FGF receptors together with the coreceptor β-Klotho to increase glucose uptake in fat cells and improve insulin sensitivity. FGF21 is widely studied in energy metabolism, obesity and metabolic disease.
| Target | Fibroblast growth factor 21 (gene FGF21; UniProt Q9NSA1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 22.3 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human placenta tissue, rat spleen tissue, rat liver tissue, rat RH35 cells, rat brain tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human FGF21 fragment (His29–Ser209), expressed in E. coli. Sequence identity with the mouse ortholog: 80.7%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes fibroblast growth factor 21 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kyong Joo Lee et al. (2018) Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways. Gut and Liver. 10.5009/gnl17443
Quan Li et al. (2020) Ethyl acetate extract of sappanwood alleviates experimental atherosclerosis in rats through changes in FGF21 and SREBP-2 expression. International Journal of Clinical and Experimental Pathology. PMID: 32211102
