| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;INCAM-100;CD106;VCAM1;L1CAM; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human FHIT |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-FHIT antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24F24; isotype IgG; reactivity: Human,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-FHIT Rabbit Monoclonal Antibody catalog # M01200-3. Tested in WB application. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: FHIT (Vascular cell adhesion protein 1).
- Antibody format: Monoclonal; clone 24F24; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
FHIT (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. Reported cellular localization context: Membrane; Single-pass type I membrane protein. Tissue expression notes (as provided): Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cancer,Cardiovascular,Cell Adhesion,Cell Adhesion Molecules,Cytoskeleton/ECM,Invasion/Microenvironment,Mesenchymal Stem Cells,Neural Signal Transduction,Neurology Process,Neuroscience,Signal Transduction,Stem Cells,Surface Molecules,Vascular Inflammation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate FHIT antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect FHIT expression by Western blot in cell or tissue lysates, Compare relative FHIT levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 17 kDa; calculated MW: 81276 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 17 kDa
- Cellular localization (provided): Membrane; Single-pass type I membrane protein.
- Tissue details (provided): Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
