| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Chromogranin-A;CgA;Pituitary secretory protein I;SP-I;Vasostatin-1;Vasostatin I;Vasostatin-2;Vasostatin II;EA-92;ES-43;Pancreastatin;SS-18;WA-8;WE-14;LF-19;Catestatin ;SL21 ;AL-11;GV-19;GR-44;ER-37;GE-25 ;Serpinin-RRG;Serpinin;p-Glu serpinin precursor;CHGA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human FHL1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-FHL1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFCI-6; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-FHL1 Monoclonal Antibody catalog # M01258. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: FHL1 (Chromogranin-A).
- Antibody format: Monoclonal; clone AFCI-6; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
FHL1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Reported cellular localization context: Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle membrane . Secreted. Associated with the secretory granule membrane through interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. . Tissue expression notes (as provided): GE-25 is found in the brain. .
Research relevance and current trends
- Research context keywords from the source record include: Cardiovascular,Domain Families,Epigenetics and Nuclear Signaling,Hypertrophy,Transcription,Zinc Finger.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate FHL1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect FHL1 expression by Western blot in cell or tissue lysates, Detect FHL1 in FFPE tissue sections by immunohistochemistry, Enrich FHL1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 85 kDa; calculated MW: 50688 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 85 kDa
- Cellular localization (provided): Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle membrane . Secreted. Associated with the secretory granule membrane through interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. .
- Tissue details (provided): GE-25 is found in the brain. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
