| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibronectin;FN;Cold-insoluble globulin;CIG;Anastellin;Ugl-Y1;Ugl-Y2;Ugl-Y3;FN1;FN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Fibronectin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EID-6 is a rabbit monoclonal antibody that recognizes fibronectin (gene FN1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 272.3 kDa, and the supplier reports an observed band at about 263 kDa.
Fibronectin (FN1) is a large extracellular matrix glycoprotein that binds integrins, collagen, fibrin and heparin. It exists as a soluble plasma form and as insoluble fibrils in tissues, and it supports cell adhesion, migration, wound healing and embryonic development. Fibronectin is a common matrix marker in studies of fibrosis and tissue repair.
| Target | Fibronectin (gene FN1; UniProt P02751, human) |
|---|---|
| Clone | EID-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 272.3 kDa |
| Observed MW | 263 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human fibronectin.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes fibronectin as a secreted protein. Tissue expression noted by UniProt (human): Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Zhifeng Yang et al. (2025) Astragalus mongholicusbunge and Angelica sinensis botanical drug decoction mitigates lung inflammation through NOX4/TGF-β1/SMAD3 signaling. Frontiers in Pharmacology. 10.3389/fphar.2025.1565569
Yuehong Gong et al. (2024) Harmine inhibits pulmonary fibrosis through regulating DNA damage repair-related genes and activation of TP53-Gadd45α pathway. International Immunopharmacology. 10.1016/j.intimp.2024.112542
Liu Mian et al. (2025) FN1 shapes the behavior of papillary thyroid carcinoma through alternative splicing of EDB region. Scientific Reports. 10.1038/s41598-024-83369-5
Yueling Tang et al. (2023) Role and mechanism of Circ-PDE7B in the formation of keloid. International Wound Journal. 10.1111/iwj.14269
