| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Insulin gene enhancer protein ISL-1;Islet-1;ISL1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Fibulin 1/FBLN1 recombinant protein (Position: D30-F703). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Fibulin 1/FBLN1 Antibody Picoband® is an antibody for FBLN1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: FBLN1 (Insulin gene enhancer protein ISL-1); UniProt: P23142
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 90 kDa, calculated 39036 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Fibulin 1/FBLN1 Antibody Picoband® catalog # A02970-1.
Biological background
Biological context: Binds and regulates the promoters of the insulin, glucagon and somatostatin genes. Involved in the specificarion of motor neurons in cooperation with LHX3 and LDB1 (By similarity). .
Expression and localization notes: cellular localization: Nucleus., tissue context: Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain..
Common research applications
- Western blotting (WB): Compare FBLN1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of FBLN1 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify FBLN1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: FBLN1 is the gene encoding fibulin-1, an extracellular matrix and plasma protein. Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Nucleus.
- Tissue details: Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
- Research category: Atherosclerosis,Cardiogenesis,Cardiovascular,Cell Type Marker,Developmental Biology,Diabetes-associated,Intracellular,Lineage Markers,Lineage Specification,Neural Stem Cells,Neurogenesis,Neurology Process,Neuron Marker,Neuroscience,Organogenesis,Stem Cells,Transcription Factors/Regulators
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
